---

• nProteomics - Studies of the Transcriptome and Proteome
• nTranscriptome - the mRNA content of a cell
• nreflects the overall pattern of gene expression in that cell

  

  http://www.scq.ubc.ca/wp-content/uploads/2006/08/Proteomics.gif

nAnalysis Methods:

Useful Tools / Techniques

• ntwo-dimensional electrophoresis
• ncomparison of two gels made of two different proteomes
• npurify a particular spot on gel --> digest with protease--> mass spectrometry --> acquire amino acid composition to support identification of genes

Hybridization analysis

• ncan detect not only expression patterns but also changes in expression patterns under two different conditions

 

Microarray (cDNA) Analysis

• n6000 genes of yeast can be spotted onto glass slides in arrays of 80 spots X 80 spots
• nmRNA extraction ( cDNA synthesis and fluorescence labeling ( assess hybridization via microarrays and  confocal microscopy)

http://employees.csbsju.edu/hjakubowski/classes/ch331/bind/microarray.gif

n

DNA Chips

• noligonucleotides are synthesized directly on the surface of the "chip"
• ndensity of 1 million per cm2 (much higher) - great throughput for screening numerous samples

 

---

Studying the Proteome

• Proteome the protein content of a cell (the entire collection of proteins in a cell)
• nreflects its biochemical capability and is the outcome of functional gene expression
• na single mRNA can give rise to more than one protein because of post-translational processing

 

Useful Tools / Techniques

• ntwo-dimensional electrophoresis
• ncomparison of two gels made of two different proteomes
• npurify a particular spot on gel --> digest with protease--> mass spectrometry --> acquire amino acid composition to support identification of genes

---